Bovine Pulmonary Artery Cell Mitochondria and Actin

FITC-TRITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with MitoTracker Red CMXRos and BODIPY FL phallacidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for BODIPY FL are 503 and 512 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission). Note the absence of signal from the blue (DAPI) fluorophore, but the bright yellow-orange fluorescence exhibited by the tubular mitochondria and the green emission from actin filaments in the cytoplasm.