Bovine Pulmonary Artery Endothelial (BPAE) Cells with MitoTracker Deep Red 633

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with MitoTracker Deep Red 633, which targets the intracellular mitochondrial network. The visible light absorption maximum of MitoTracker Deep Red 633 is 644 nanometers and the emission maximum occurs at 665 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the tubular mitochondrial network that extends throughout the cytoplasm.