Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)

The 3T3 cell culture appearing in the digital image above was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3. The fibroblast cells were also stained with DAPI, which preferentially binds to nuclear DNA, and Alexa Fluor 488 conjugated to phalloidin to target the F-actin network. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.