Fern Rhizome Thin Section

B-3A Longpass Emission (Wide Bandwidth Excitation) Blue Set

Autofluorescence emission intensity from a thin section of fern rhizome (Cyrtomium falcatum) tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the blue region, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 420 and 460 nanometers. Note the presence of spectral bleed-through from autofluorescence emission in the yellow and red spectral regions with the B-3A filter combination.