Human Fetal Lung Fibroblast Cells (MRC-5 Line)

The mitochondria present in the culture of MRC-5 fibroblasts featured in the digital image above were targeted with MitoTracker Red CMXRos, a derivative of X-rosamine. In addition, the culture was labeled for nuclear DNA and the cytoskeletal F-actin network with SYTOX Green and Alexa Fluor 633 conjugated to phalloidin, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, instead of individual image channels being pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, Alexa Fluor 633 was pseudocolored purple, MitoTracker Red CMXRos was pseudocolored green, and SYTOX Green was pseudocolored cyan.