Human Lung Carcinoma Cell Mitochondria with Cy2

YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set

Fluorescence emission intensity from a culture of human lung carcinoma epithelial cells (A-549 line) that were immunofluorescently labeled with primary anti-oxphos complex V inhibitor protein monoclonal antibodies (mouse) followed by goat anti-mouse Fab fragments conjugated to Cy2. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. Note the high level of green fluorescence from the labeled intracellular mitochondria, but the absence of detected emission from the blue and orange fluorophores (DAPI and Alexa Fluor 568) targeting the nuclei and actin, respectively.