Human Lung Cells with DAPI, Alexa Fluor 488, and MitoTracker Red CMXRos

Fluorescence emission intensity from a culture of human lung cells (MRC-5 line) stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nucleus, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen.