Indian Muntjac Actin Cytoskeleton with Alexa Fluor 532

G-2E/C Bandpass Emission (Narrow Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells stained with Alexa Fluor 532 conjugated to phalloidin, which binds to the intracellular filamentous actin network. The visible light absorption maximum of Alexa Fluor 532 is 531 nanometers and the emission maximum occurs at 554 nanometers in the green-yellow region of the spectrum. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and MitoTracker Green FM (targeting mitochondria; green emission). Note the absence of signal from the green (MitoTracker) and blue (DAPI) fluorophores, which are not efficiently excited at green wavelengths, but the bright yellow-orange fluorescence exhibited by the actin filaments.