Lysosomes in Distressed Cells with LysoTracker Red

G-2E/C Bandpass Emission (Narrow Bandwidth Excitation) Green Set

Fluorescence emission intensity from a stressed culture of Indian Muntjac deerskin fibroblast cells adherent on a cover glass. Nuclei in the cells were targeted with the nucleic acid probe SYTOX Green, which has an excitation maximum at 504 nanometers and an emission maximum at 523 nanometers when bound to DNA in cultured cells and tissue sections. In addition, the cells were stained with LysoTracker Red DND-99 (targeting lysosomes; excitation at 577 nanometers and emission at 590 nanometers) and Alexa Fluor 350 conjugated to phalloidin (targeting actin; excitation at 346 nanometers and emission at 442 nanometers). Note the absence of significant emission intensity from the blue and green fluorophores, but the high level of fluorescence from lysosomes that surround the nucleus.