Indian Muntjac Skin Cell Tubulin with Fluorescein

YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe Hoechst 33258, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. The visible light absorption maximum of fluorescein is 490 nanometers and the emission maximum occurs at 525 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the high level of green fluorescence observed from the intracellular microtubule network that extends throughout the cytoplasm, but an absence of signal from the blue and orange fluorophores (Hoechst and Alexa Fluor) targeting the nuclei and actin, respectively.