Mouse Intestine Tissue Section with SYTOX Green and Alexa Fluor 568

FITC-Texas Red Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a thin section of mouse intestine stained with Alexa Fluor 350 wheat germ agglutinin, a blue fluorescent lectin that is specific to the mucus of goblet cells. In addition, the specimen was simultaneously stained with Alexa Fluor 568 phalloidin (filamentous actin; 600 nanometer emission) and SYTOX Green (nuclei; 504 nanometer excitation and 523 nanometer emission). Note the absence of signal from the blue fluorophore (Alexa Fluor 350), but the bright green fluorescence of nuclei in the tissue specimen due to SYTOX Green, and the orange-red fluorescence from actin filaments in the brush border.