Rat Liver Thin Section with SYTOX Green

YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set

Fluorescence emission intensity from a thin section of rat liver stained with SYTOX Green for nuclear DNA. In addition, the specimen was simultaneously stained for filamentous actin with Alexa Fluor 546 conjugated to phalloidin, and with Alexa Fluor 350 wheat germ agglutinin (WGA), a blue fluorescent lectin conjugate that is specific to the mucus of goblet cells. The visible light absorption maximum of SYTOX Green, when bound to DNA, is 504 nanometers and the emission maximum occurs at 523 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the strong green fluorescence emission from labeled DNA in the cell nuclei, while no signal is observed from the blue and yellow fluorophores (Alexa Fluor 350 and Alexa Fluor 546) targeting glycoproteins and actin, respectively.