Sheep Stomach Tissue Section with SYTOX Orange and Oregon Green 488

Fluorescence emission intensity from a thin section of sheep stomach tissue stained for DNA and glycoproteins with SYTOX Orange and Oregon Green 488 wheat germ agglutinin, respectively. The visible light absorption maximum of SYTOX Orange is 547 nanometers and the emission maximum occurs at 570 nanometers, while the corresponding values for Oregon Green 488 are 496 and 524 nanometers. In addition, the specimen was simultaneously stained with Alexa Fluor 350 conjugated to phalloidin (targeting the actin network; blue emission). Note the absence of signal from the blue (Alexa Fluor 350) fluorophore, but the bright orange-red fluorescence exhibited by the nuclei and the segregated green emission from glycoproteins in the tissue section.