Swiss Mouse (3T3) Cells with DAPI and Alexa Fluor 488

DAPI-FITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of Swiss mouse (3T3 line) cells that were immunofluorescently labeled with primary anti-cytochrome oxidase mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 568. The specimen was also labeled with several additional probes, including lectin Helix pomatiaagglutinin (HPA) conjugated to Alexa Fluor 488 (green emission), which selectively binds to glycoproteins in the Golgi apparatus in this cell line. Cytoskeletal actin filaments were stained with Alexa Fluor 680 (red excitation; near-infrared emission) and the nuclear DNA was labeled with DAPI (blue emission). Note the prominent green staining of the Golgi apparatus and the blue fluorescence intensity observed in the nuclei. Fluorescence emission in the orange and red regions is excluded by the DAPI-FITC filter set, so the 568 and 680 Alexa Fluor dyes are not visible in the image presented above.